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Search through our support documentation and FAQs for ArcherDX, now Invitae, products and services.

CNV Detection and Analysis Using Invitae’s Anchored Multiplex PCR Chemistry

Archer® Analysis version 7.0 includes an updated copy number variation (CNV) algorithm within the DNA pipeline. This tech note describes the updated algorithm along with best practices for detecting CNVs using Invitae’s Anchored Multiplex PCR (AMP)-based platform.

White Paper: Invitae Micosatellite Instability Detection

Invitae’s VariantPlex® panels are a collection of four pan-solid tumor assays that use next-generation sequencing powered by Anchored Multiplex PCR (AMP™) with a modular approach to identify microsatellite instability status with high sensitivity and specificity without a matched normal sample.

White Paper: Archer® Analysis Data Security

Archer Analysis software was designed with data security in mind, delivering customers a reliable analysis platform that couples comprehensive regulatory compliance with stringent data protection mechanisms.

VariantPlex® Myeloid NGS panel validation study

Hematologic malignancies, including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and myeloproliferative neoplasms (MPN) are oligoclonal disorders caused by germline or acquired genetic abnormalities in hematopoietic cells. Testing based on targeted next-generation sequencing (NGS) of the genes associated with these disorders can help identify somatic mutations.

Analytical and Methodological Validation of the LiquidPlex™ Assay

We conducted an analytical and methodological validation of the LiquidPlex™ assay for solid tumors. This next generation sequencing (NGS) assay is designed to identify variants within circulating tumor DNA (ctDNA) following extraction from the plasma fraction of blood. While the genomic targets covered by the assay are broad, the scope of this validation includes 58 genomic positions across 25 genes.

The Use of Molecular Barcodes in Anchored Multiplex PCR

Sample barcoding, also called sample indexing, is a common approach to labeling samples for multiplex sequencing and analysis. All nucleic acids in a sample are labeled with the same sequence tag, and the resulting library is pooled with other libraries and sequenced in parallel in a single run. Then, during analysis, the sample-specific indexes enable the software to separate the multiplexed sequence data in sample-specific data sets.

Detection of Internal Tandem Duplications in FLT3 with Anchored Multiplex PCR and next-generation sequencing (NGS)

FLT3 encodes a receptor tyrosine kinase that is involved in p53 activation and has roles in cell growth arrest and apoptosis. Internal tandem duplications (ITDs) in the juxtamembrane domain result in constitutive activation of FLT3, causing aberrant cell growth leading to tumorigenesis. FLT3-ITDs are associated with poor prognosis of acute myeloid leukemia (AML) and are detected in about 25% of AML cases. As FLT3-ITD expressed kinases are sensitive to tyrosine kinase inhibitors, they are of considerable interest for the development of novel AML treatments.

Comprehensive Report on FFPE Extraction Methods

Six popular commercially-available FFPE extraction panels are tested for performance in conjunction with Anchored Multiplex PCR (AMP™) chemistry.

95 MDAF: Modeling Noise in NGS Data with Per-Base Resolution for Statistically Informed, Position-Dependent Variant Calling

Archer® Analysis expresses LOD as the minimum allele fraction at which a variant can be distinguished from underlying noise at a statistical power of 0.95, referred to as 95 MDAF. This enables precise detection of sensitivity at each base position.

95 MDAF: Modeling Noise in NGS Data with Per-Base Resolution for Statistically Informed, Position-Dependent Variant Calling

Archer® Analysis expresses LOD as the minimum allele fraction at which a variant can be distinguished from underlying noise at a statistical power of 0.95, referred to as 95 MDAF. This enables precise detection of sensitivity at each base position.

Comprehensive Report on FFPE Extraction Methods

Six popular commercially-available FFPE extraction panels are tested for performance in conjunction with Anchored Multiplex PCR (AMP™) chemistry.

Detection of Internal Tandem Duplications in FLT3 with Anchored Multiplex PCR and next-generation sequencing (NGS)

FLT3 encodes a receptor tyrosine kinase that is involved in p53 activation and has roles in cell growth arrest and apoptosis. Internal tandem duplications (ITDs) in the juxtamembrane domain result in constitutive activation of FLT3, causing aberrant cell growth leading to tumorigenesis. FLT3-ITDs are associated with poor prognosis of acute myeloid leukemia (AML) and are detected in about 25% of AML cases. As FLT3-ITD expressed kinases are sensitive to tyrosine kinase inhibitors, they are of considerable interest for the development of novel AML treatments.

The Use of Molecular Barcodes in Anchored Multiplex PCR

Sample barcoding, also called sample indexing, is a common approach to labeling samples for multiplex sequencing and analysis. All nucleic acids in a sample are labeled with the same sequence tag, and the resulting library is pooled with other libraries and sequenced in parallel in a single run. Then, during analysis, the sample-specific indexes enable the software to separate the multiplexed sequence data in sample-specific data sets.

Analytical and Methodological Validation of the LiquidPlex™ Assay

We conducted an analytical and methodological validation of the LiquidPlex™ assay for solid tumors. This next generation sequencing (NGS) assay is designed to identify variants within circulating tumor DNA (ctDNA) following extraction from the plasma fraction of blood. While the genomic targets covered by the assay are broad, the scope of this validation includes 58 genomic positions across 25 genes.

VariantPlex® Myeloid NGS panel validation study

Hematologic malignancies, including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and myeloproliferative neoplasms (MPN) are oligoclonal disorders caused by germline or acquired genetic abnormalities in hematopoietic cells. Testing based on targeted next-generation sequencing (NGS) of the genes associated with these disorders can help identify somatic mutations.

White Paper: Archer® Analysis Data Security

Archer Analysis software was designed with data security in mind, delivering customers a reliable analysis platform that couples comprehensive regulatory compliance with stringent data protection mechanisms.

White Paper: Invitae Micosatellite Instability Detection

Invitae’s VariantPlex® panels are a collection of four pan-solid tumor assays that use next-generation sequencing powered by Anchored Multiplex PCR (AMP™) with a modular approach to identify microsatellite instability status with high sensitivity and specificity without a matched normal sample

 

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